'Home-brew' morphine from brewer's yeast now possible -study

By Sharon Begley

NEW YORK (Reuters) - Home-brewing could soon take on a more dangerous twist: Scientists have engineered brewer's yeast to synthesize opioids such as codeine and morphine from a common sugar, an international team reported on Monday.

"It is going to be possible to 'home-brew' opiates in the near future," Dr. Christopher Voight of the Massachusetts Institute of Technology, who was not involved in the research, told reporters.

The process described in Nature Chemical Biology is inefficient, requiring 300 liters of genetically engineered yeast to produce a single 30 milligram dose of morphine.

But with improvements that are well within reach, that dose could be obtained from "a glass of yeast culture grown with sugar on a windowsill," Dr. Voight said.

The molecular structure of morphine and other opioids is so complex, they are isolated or manufactured from compounds in plants, such as opium poppies. Chemists have never been able to produce them from off-the-shelf components.

Scientists, however, recently reported engineering yeast to carry out the second part of the 15-step opioid-producing reaction. What remained was just the hurdle of coaxing yeast to carry out the first part.

That is what scientists, led by Dr. John Dueber of the University of California, Berkeley, have accomplished.

They isolated a crucial enzyme from sugar beets, mutated its gene to make it more productive, and slipped it into yeast.

They then added more foreign DNA and achieved their goal: the yeast carried out the first half of the reaction that produces opioids.

Combined with the earlier recipe for the rest of the reaction, said Dr. Dueber, "it's now a matter of linking (the steps) together and scaling up the process."

Outside scientists agreed, and expressed alarm over that prospect.

Although additional DNA tweaks could produce yeast that synthesize cheaper, less addictive, and more effective pain-killers, the creation of morphine-making yeast could dramatically increase access to illegal opiates, which have caused a scourge of addiction in many communities.

The study, which the authors alerted colleagues to before publication, could make illegal drugs "easy to grow, conceal and distribute," with little more than a home-brew beer-making kit, policy analysts warned in an accompanying commentary.

The analysts called for policies to regulate engineered-yeast strains, such as confining them to licensed facilities.

But the cat may be out of the bag. With the recipe for opiate-producing yeast now public, Dr. Dueber said, "anyone trained in basic molecular biology could theoretically build it."

SOURCE: http://bit.ly/1Kgp66u

Nat Chem Biol 2015.

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